Hanahan's Broth (SOB Medium) was developed by Douglas Hanahan (1983) for the cultivation of competent Escherichia coli cells prior to transformation.
SOB Medium is a nutrient-rich, bacterial growth medium that is used for microbiological culture, generally of E. coli.
When 20 mM (0.36%) D-glucose is added to this nutritionally rich medium it is referred to as SOC (Super Optimal broth with Catabolite repression) Medium. It is used in the final stage of transformation; this allows the E. coli cells to mend the perforations and replicate.
Synonyms: Super Optimal Broth; SOB
|Solutions Available||S657 - SOC Medium, 1X (H289 + 20 mM glucose)|
|Grams of powder to prepare 1 Liter||28.09|
|Synonyms||Super Optimal Broth; SOB|
|Storage Temp.||2 to 8 °C|