King's B Medium

Yeast Extract Beef (YEB) Broth

Yeast Extract Beef (YEB) Broth

Hanahan's Broth (SOB Medium)

Product ID
H289
Grouped product items
Package Size Qty
1 L
$6.78
100 g
$20.55
500 g
$78.69

Hanahan's Broth (SOB Medium) was developed by Douglas Hanahan (1983) for the cultivation of competent Escherichia coli cells prior to transformation.

SOB Medium is a nutrient-rich, bacterial growth medium that is used for microbiological culture, generally of E. coli.

When 20 mM (0.36%) D-glucose is added to this nutritionally rich medium it is referred to as SOC (Super Optimal broth with Catabolite repression) Medium. It is used in the final stage of transformation; this allows the E. coli cells to mend the perforations and replicate.

Synonyms: Super Optimal Broth; SOB

Solubility Water
Physical Form Solid
Solutions Available S657 - SOC Medium, 1X (H289 + 20 mM glucose)
Grams of powder to prepare 1 Liter 28.09
Product Number H289
Synonyms Super Optimal Broth; SOB
Weight 0.0000
Storage Temp. 2 to 8 °C
Tariff Code 3821.00.0000