Bold's Basal Medium (BBM)
Bold's Basal Medium (BBM) is a freshwater algae medium that has been used to grow a variety of green algal cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). The predominantly inorganic nature of this medium facilitates itself as an axenic-culture maintenance medium (Nichols and Bold, 1965).
Requires 1 mL/L Sulfuric Acid, 0.1% (v/v) (Product # S7664).
|Solutions Available||B1650 (50x)|
|Grams of powder to prepare 1 Liter||0.705|
|Other Notes||Add 1.0 mL of 0.1% Sulfuric Acid (S7664) to complete this medium.|
|Storage Temp.||2 to 8 °C|