Gelzan™ is produced by fermentation and an agar substitute also known as Gellan Gum. Use at 2-4 g/L. Do not use with media containing high levels of calcium such as DKW and Quoirin & Lepoivre. Also if divalent cation (Ca2+, Mg2+) concentrations are below that of 1/2X MS (e.g., our Orchid Maintenance Medium with Charcoal and without Agar) you may need to use 10-12 g/L to obtain free-standing gels. This gelling agent requires melting at 121°C. If your autoclave doesn't reach that temperature for 15-20 min the final gel may appear clumpy.
Note: Kanamycin is known to lose activity when used with gellan gums. We DO NOT recommend its use with gellan gums.
Product of CP Kelco.
|Solubility||Heated to Boiling Water|
|Other Notes||Plant Tissue Culture Tested|
|Gelling Strength||400-700 g/cm2|
|Storage Temp.||Room Temperature|