Kao & Michayluk Basal Salt Mixture
Contains the macro- and micronutrients as described by Kao & Michayluk (1975).
This medium was originally developed for the culture of Vicia hajastana protoplasts. The culture of low cell densities (1-2 cells/mL) is enhanced when the medium is supplemented with organic acids, sugar alcohols, sugars, plant growth regulators and amino acids. (See K427) Cell growth and division could also be enhanced by raising the concentrations of Calcium Chloride from 1 to 5 mM.